Generator
AntiFreeze

Part:BBa_K193209:Design

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)


Fusion protein (TmTHP - EGFP) as Anti-Freeze Protein regulated by LacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1102
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1102
    Illegal NheI site found at 1120
    Illegal NotI site found at 1132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1102
    Illegal BglII site found at 43
    Illegal BamHI site found at 637
    Illegal XhoI site found at 538
    Illegal XhoI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1102
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1102
    Illegal AgeI site found at 847
    Illegal AgeI site found at 886
    Illegal AgeI site found at 958
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used biobrick standard assembly.


Source

We used K193206 and K193205.

References